Coding

Part:BBa_K357003:Design

Designed by: Elena Pasko   Group: iGEM10_Toronto   (2010-06-23)

3-oxoadipate enol-lactonase (also known as beta-ketoadipate enol-lactone hydrolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contains no restriction sites. The sequence was optimized for E.Coli.

Source

SOURCE Pseudomonas putida KT2440 (genomic sequence)

AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D., 

          Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,

          Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,

          Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,

          Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,

          Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,

          Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,

          Duesterhoft,A., Tummler,B. and Fraser,C.

TITLE     Complete genome sequence and comparative analysis of the

          metabolically versatile Pseudomonas putida KT2440

JOURNAL   Environ. Microbiol. 4 (12), 799-808 (2002)


References